Chapter 9 Biotechnology and recombinant DNA

Unformatted text preview: Chapter 10: Recombinant DNA Technology, Plant Biotechnology, and Genomics Multiple-Choice Questions 1. Introduction; p. 192; easy; ans: d Traditional breeding programs: a. involve inserting a plasmid into a host cell. b. involve infecting a host plant with a virus. c. allow gene transfers between species incapable of hybridizing. d. depend on inherent genetic variability. e. allow the precise insertion of genes into a plant. 2. Recombinant DNA Technology; p. 192; easy; ans: c Genetically modified plants were first grown commercially in: a. 1965. b. 1985. c. 1996. d. 2001. e. 2009. 3. Recombinant DNA Technology; p. 193; moderate; ans: c Which of the following statements concerning restriction enzymes is FALSE? a. They are a type of endonuclease. b. They recognize DNA sequences typically four to six nucleotides long. c. They recognize single-stranded DNA sequences. d. Some make straight cuts in the DNA. e. They recognize specific palindromic sequences. 4. Recombinant DNA Technology; p. 194; moderate; ans: a A sticky-ended fragment can join with another DNA segment only if that segment: a. has been cut with the same restriction enzyme. b. has been cut with the same ligase. c. is palindromic. d. is part of a plasmid. e. is part of a virus. 5. Recombinant DNA Technology; p. 194; easy; ans: e DNA ligase would most likely be used in recombinant DNA technology to: a. function as a vector. b. screen for recombinant plasmids. c. stimulate a transformed cell to divide. d. cut DNA into fragments with sticky ends. e. join DNA fragments together. 6. Recombinant DNA Technology; pp. 194-195; easy; ans: e Which of the following statements concerning vectors is FALSE? a. Vectors are used to transform cells. b. Vectors function as DNA carriers. c. A virus can be a vector. d. A plasmid can be a vector. e. A host cell can be a vector. 7. Recombinant DNA Technology; p. 195; difficult; ans: b By definition, a transformed cell: a. contains a plasmid. b. contains a recombinant DNA molecule. c. contains a virus. d. functions as a vector. e. divides in such a way so as to amplify the DNA fragment. 8. Recombinant DNA Technology; p. 195 moderate; ans: c The amp R gene is used...

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